Capping B (THF / pyridine / N-methylimidazole; V / V / V = 80:10:10)

Description

Capping B Solution – Tetrahydrofuran : Pyridine : N-Methylimidazole (80:10:10 v/v/v)

This Capping Reagent B is a three-component solution composed of tetrahydrofuran (THF), pyridine, and N-methylimidazole, mixed in an 80:10:10 volume ratio. It is used in the capping step of solid-phase oligonucleotide synthesis to activate Capping Reagent A (acetic anhydride), enabling acetylation of unreacted 5′-hydroxyl groups.

• Composition: THF : Pyridine : N-methylimidazole (80:10:10 v/v/v)

• Function: Activates Capping Reagent A for effective 5’-OH capping

• Application: Capping solution for DNA/RNA synthesis workflows

• Instrument Compatibility: Suitable for most automated synthesizers

• Formulation: Ready-to-use

This formulation provides a more solvent-rich, low-viscosity matrix compared to traditional acetonitrile-based capping reagents, which may be advantageous for certain synthesis protocols or platforms.

References

• Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine O6 Dephosphitylation Strategy. Nature Communications Chemistry 2025