6-Carboxyfluorescein NHS ester, single isomer (6-FAM-SE)

Description

6-Carboxyfluorescein Single Isomer (NHS ester, 6-FAM-SE)

Overview
6-Carboxyfluorescein single isomer NHS ester (6-FAM-SE) is an amine-reactive fluorescent dye commonly used for high-resolution labeling of biomolecules. This product is supplied as a pure single isomer of 6-carboxyfluorescein, providing enhanced reproducibility in sensitive labeling applications. The NHS ester reacts with primary amines on biomolecules, forming stable amide bonds.

Applications

• Precise oligonucleotide labeling for qPCR, probes, and hybridization assays

• Antibody labeling for imaging and flow cytometry

• Protein or peptide modification for fluorescence-based detection

Use Considerations
This single-isomer formulation ensures site-specificity and batch-to-batch consistency, making it ideal for critical assays where mixed isomers may introduce variability. NHS ester chemistry is fast and efficient under mild conditions and widely used for labeling amine-functionalized oligonucleotides and proteins.

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