Equalbit dsDNA Broad Range Assay Kit

Description

The Equalbit dsDNA BR Assay Kit is a simple, accurate, and broad-range fluorescence-based kit for dsDNA quantification using Qubit fluorometers—ideal for diverse research needs.

Equalbit dsDNA BR Assay Kit Features & Components

• Complete set includes fluorescence reagents, buffer, and dsDNA standards.
• High dsDNA specificity—not influenced by RNA.
• Linear quantification range: 4–2000 ng total dsDNA.
• Excellent tolerance to common contaminants: salts, free nucleotides, proteins, solvents, detergents.
• Operates at room temperature: dilute reagent, add sample, and measure with Qubit.

Assay Protocol

• Dilute fluorescence reagent in buffer to create working solution.
• Add dsDNA standards or samples.
• Incubate briefly (~2 min) at room temperature, protected from light.
• Measure fluorescence using Qubit’s “dsDNA BR” program.

Established Applications

• NGS library prep QC: verify dsDNA concentration before sequencing.
• PCR/plasmid quantification: confirm input DNA amount for cloning.
• Environmental & clinical DNA assays: robust to extraction contaminants.

Supplied by You Do Bio’s supplier Vazyme Biotech.