ONPG – Galactosidase Assay Kit

Description

The ONPG Galactosidase Assay Kit offers an optimized, stable, and highly convenient solution for accurately measuring active β-galactosidase levels in cells transfected with Lac Z expressing plasmids. The Lac Z gene is widely utilized as a reporter gene in various experiments due to β-galactosidase’s remarkable resistance to proteolytic degradation and its easily quantifiable activity.

This assay operates on a simple yet effective principle: β-galactosidase catalyzes the hydrolysis of orthonitrophenyl-β-D-galactopyranoside (ONPG) into ortho-nitrophenol (ONP). This reaction produces a distinct bright yellow color, which can be detected and measured spectrophotometrically at 420 nm. The intensity of the resulting color is directly proportional to the concentration of β-galactosidase present in your sample, ensuring reliable and quantitative results.

Key Applications of the ONPG Galactosidase Assay:

• Reporter Gene Assays: Widely used to study gene expression, promoter/enhancer activity, and to monitor transfection efficiency in mammalian, yeast, and bacterial cells.
• Bacterial Identification & Characterization: Essential for differentiating various microorganisms, including members of Enterobacteriaceae, based on their β-D-galactosidase activity, and for distinguishing late lactose fermenters from non-lactose fermenters.
• Quantitative Enzyme Activity Measurement: Enables the precise quantification of β-galactosidase activity in diverse sample types such as cell lysates, plasma, serum, and other biological fluids, valuable for enzyme kinetics and diagnostic research.

This kit includes all necessary components for accurate measurement: ONGP Substrate solution, β-mercaptoethanol, Lysis Buffer, Assay Buffer, Stop Solution, and β-galactosidase enzyme. Please note, this product is developed, designed, and sold exclusively for Research Use Only (RUO) and for in vitro use. It has not been tested for diagnostic or drug development purposes and is not suitable for administration to humans or animals.

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