PBS Tween 20 pH 7.6

Description

PBS Tween pH 7.6 (PBST) is a high-quality, molecular biology grade buffer designed for reliable and convenient use across a wide range of applications. This water-based salt solution, also known as Phosphate-buffered Saline with Tween™-20 (PBS-T), is ideal for various laboratory procedures. It is particularly effective for sample preparation and as a wash buffer in general immunoassay applications.

The key component, Tween 20, is a non-ionic detergent additive. It plays a crucial role in reducing non-specific binding and minimizing protein-protein interactions during the wash step in protein and immunoassay procedures. By decreasing non-specific binding and staining, PBS-T with Tween 20 makes the interpretation of results from techniques like ELISA and Western blotting significantly easier and more reliable.

Key applications of PBS with Tween™ 20 (pH 7.6) include:

• • Wash Buffer in Immunolabelling Techniques: Essential for procedures such as ELISA and Western Blotting, ensuring clean and accurate results.
  • Blocking Buffer for Plate-based Assays: Helps prevent non-specific binding to microplates, improving assay specificity.
  • Protein-Plate Coating: Used to facilitate the uniform coating of proteins onto surfaces for various assays.