PickMutant Site-directed Mutagenesis Kit

Description

PickMutant™ Site-directed Mutagenesis Kit

The PickMutant™ Site-directed Mutagenesis Kit by Canvax is a reliable, robust, and highly efficient PCR-based mutagenesis solution. This kit simplifies the creation of single or multiple point mutations, deletions, or insertions through a rapid, straightforward, one-step protocol, completing in less than 3 hours.

Key Features:

• High Effectiveness: Achieves various types of mutations with precision.
• Broad Compatibility: Works seamlessly with any bacterial strains or primers.
• Versatility: Compatible with any cloning vector.
• Efficiency: Includes the highly efficient pSpark® vector for cloning blunt fragments.
• Robustness: Allows for simultaneous assembly and cloning of PCR fragments.

Product Specifications:

• Size: 15 reactions
• Includes:
  • 150 μL Proofreading DNA Polymerase MasterMix (2x)
  • 300 U Glue Enzyme (10 U/μL)
  • 40 μL Glue Enzyme Buffer (10x)
  • 5 μL Insert Control DNA
  • 15 μL pSpark® I (20 ng/μL)
• Storage: -20 ºC (NON Frost-Free Freezer), shipped in a gel pack.
• Usage: Developed, designed, and sold exclusively for research purposes and in vitro use only. Not tested or suitable for diagnostics or drug development, or administration to humans or animals.

Established Applications of Site-Directed Mutagenesis Technology:

This technology is widely used in molecular biology and genetic engineering for:

1. Studying Protein Function: By altering specific amino acids, researchers can investigate their roles in protein activity, structure, and interactions.
2. Identifying Enzyme Active Sites: Precise mutations help pinpoint critical residues involved in an enzyme’s catalytic mechanism.
3. Designing New Proteins: Enables the engineering of proteins with enhanced or novel functions, such as improved stability or altered substrate specificity.

See our other Mutagenesis Kits