TBS Buffer pH 7.4 (10X)

Description

TBS Buffer pH 7.4 (10x) is a high-quality, reliable Tris Buffered Saline solution designed to maintain pH within a tight physiological range, ensuring reproducible results in various biological applications. This 10x concentrated buffer is isotonic and non-toxic to cells, effectively mimicking physiological conditions vital for sensitive assays.

The stability and consistency of this buffer make it an indispensable tool in molecular biology and immunology laboratories. Its ability to dilute substances and act as an effective washing agent helps reduce non-specific binding, leading to cleaner and more accurate experimental outcomes.

Key applications of TBS Buffer pH 7.4 (10x) include:

• Washing Buffer in Immunoassays: Widely used in techniques such as ELISA and Western Blotting to remove unbound antibodies and minimize background noise.
• Immunohistochemistry Staining: Essential for cleaning the background during immunohistochemistry procedures, enhancing signal clarity.
• Dilution of Antibodies: Used to dilute phosphatase and peroxidase conjugated antibodies in Western Blotting, ensuring optimal antibody concentration for detection.

References

• Microphysiological sensing platform for an in-situ detection of tissue-secreted cytokines. Biosensors and Bioelectronics: X, 2019.