Tin DNA polymerase Isothermal Mastermix (ISO-001Tin) – 300 reactions

Description

Tin(exo-) LF DNA Polymerase

 

• OptiGene’s patented thermostable DNA polymerase for amplification reactions requiring an initial heat-denaturing step.
• Extreme thermostability and strong strand-displacement activity enable applications unachievable in a single, closed-tube reaction using alternative isothermal enzymes.

 

Key features:

 

• Extreme thermostability* ( < 98ºC )
• Superior strand-displacement activity
• 65ºC temperature optimum
• 5’-3’ exonuclease minus
• 3’-5’ exonuclease minus

 

*no loss of activity is observed in a LAMP reaction following 5 minutes @ 95ºC step.

Advantages of HD-LAMP:

 

• Template denaturation step at 95ºC prior to amplification has been shown to increase target sensitivity¹
• Increased primer specificity – reduces false-priming that may occur at lower temperatures.
• Simple template extraction (cell-lysis).
• Sample heat-lysis, amplification and detection in a single tube.

 

¹Mori Y, Notomi T. 2009. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.
J Infect Chemotherapy. 15(2):62-9.