Tris-Glycine Buffer pH 8.3 (10X)

Description

Tris-Glycine Buffer pH 8.3 (10x) is a high-quality, molecular biology grade buffer designed for reliable and convenient use across a wide range of applications. This Tris-Glycine (TG) solution is a premium and convenient running buffer, particularly effective in both native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins.

Prepared as a 10x concentrated stock solution, this buffer ensures consistency and efficiency in your laboratory procedures, saving valuable preparation time. Its optimized pH of 8.3 provides a stable environment crucial for accurate separation and analysis in protein-based applications.

Key applications of Tris-Glycine Buffer pH 8.3 (10x) include:

• Protein Electrophoresis: Widely used as a running buffer for both native and denaturing protein gel electrophoresis, facilitating optimal protein migration and separation.
• Polyacrylamide Gel Electrophoresis (PAGE): Essential for various PAGE techniques, including homogeneous and gradient gels, for robust and reproducible results.
• Western Blotting: Utilized in Western blotting procedures, contributing to efficient protein transfer and clear detection.