Capping B (16% N-methylimidazole in THF)

Description

Capping B Solution – 16% N-Methylimidazole in Tetrahydrofuran (84:16 v/v)

This Capping Reagent B is a ready-to-use solution composed of 16% N-methylimidazole in tetrahydrofuran (THF), mixed in an 84:16 volume ratio. It is designed for use in the capping step of solid-phase oligonucleotide synthesis, where it functions as an activator for acetic anhydride (Capping Reagent A).

• Composition: 84% THF / 16% N-methylimidazole (v/v)
• Function: Activator for Capping Reagent A
• Application: For 5’-OH acetylation during DNA/RNA synthesis
• Instrument Compatibility: Suitable for both manual and automated synthesizers
• Formulation: Ready-to-use

This formulation delivers a precise, controlled activation of the capping step and is often favored in automated or high-throughput synthesis systems due to its low viscosity and stability in THF.