Resazurin Cell Viability Assay

Description

Resazurin Cell Viability Assay

The Resazurin Cell Viability Assay is a reliable, sensitive and easy-to-use fluorescent assay that detects cellular metabolic activity. Resazurin (7-Hydroxy3H-phenoxazin-3-one 10-oxide) is a blue dye non-fluorescent until it is irreversibly reduced to the pink colored and highly red fluorescent resorufin by dehydrogenase enzymes in metabolically active cells.

The fluorescent signal is monitored using 530-560 nm excitation wavelength and 590 nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample.

Advantages and Features

• Easy procedure: easy to perform with minimal handling.
• Really convenient and fast protocol: just one step to results.
• Proven performance in a wide range of cell types, including bacteria, yeast, fungi, protozoa, and cultured mammalian cells.
• Highly Sensitive.
• Safe.
• Versatile: compatible with most used instumentation.
• Risk-free: covered by Canvax™ Quality 100% Guarantee.

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References

• A case of persistent bacteraemia by Ralstonia mannitolilytica and Ralstonia pickettii in an intensive care unit. Infection and Drug Resistance 2019
• The effect of graphene oxide on signalling of xenobiotic receptors involved in biotransformation. Chemosphere 2020.
• Hydralazine and Panobinostat Attenuate Malignant Properties of Prostate Cancer Cell Lines. Pharmaceuticals 2021.