pSpark® II Blunt-end DNA Cloning Kit

184.00

pSpark® II Blunt End DNA Cloning Kit enables high-efficiency cloning of blunt-ended DNA fragments. It is designed for fast and accurate cloning, ensuring reliable insert recovery.

Best for users who need flexible blunt-end cloning across different PCR and ligation conditions

pSpark II Blunt-End Cloning Protocol

Use our Cloning Kit Selection Guide

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SKU: C0002 Categories: ,

Canvax

Description

pSpark® II Blunt-end DNA Cloning Kit

pSpark® II Blunt End DNA Cloning Kit enables high-efficiency cloning of blunt-ended DNA fragments. It is designed for fast and accurate cloning, ensuring reliable insert recovery.

The system offers reduced background and excellent colony screening efficiency. It is ideal for researchers who require reproducible and straightforward cloning results.

Advantages & Features
  • High efficiency: >2,500 colonies with optimal inserts.
  • Reliable: <1% background, no toxic genes required.
  • Versatile: compatible with proofreading polymerases and diverse ligation protocols.
  • Robust: supports ligation times from 10 min to overnight.
  • Cost-saving: no phosphorylated primers needed.

Blunt end cloning kits Application Note

See our other blunt-end cloning kits

References

  • Molecular and biochemical analysis of XDH from Phaseolus vulgaris suggest that uric acid protects the enzyme against the inhibitory effects of nitric oxide in nodules. Plant Physiology and Biochemistry 2019.
  • Identification and Molecular Characterization of Superoxide Dismutases Isolated From A Scuticociliate Parasite: Physiological Role in Oxidative Stress. Nature Scientific Reports 2019.
  • A real-time quantitative PCR assay using hydrolysis probes for monitoring scuticociliate parasites in seawater. Aquaculture 2022.

Additional information

Weight 0.1 kg
Dimensions 10 × 10 × 10 cm
pack-size

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