pSpark® Universal DNA Cloning Kit

194.00

A versatile, universal cloning kit compatible with both blunt-end and A-tailed PCR products for reliable and efficient ligation.

pSpark – Universal Cloning Kit Protocol

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SKU: C0019 Categories: ,

Canvax

Description

Universal Cloning Kit

Versatile cloning kit compatible with both blunt-end and A-tailed PCR products for reliable and efficient ligation.

pSpark® Universal Cloning Kit offers a flexible and efficient solution for cloning both A-tailed and blunt-ended PCR products in a single system. The proprietary dual-end vector design allows direct ligation of fragments generated by either Taq or proofreading polymerases without the need for enzymatic modification or additional preparation steps.

This all-in-one kit ensures high transformation efficiency, minimal background, and stable insert maintenance, making it ideal for routine cloning, sequencing, and plasmid construction. It provides a simple and robust workflow, enabling researchers to move seamlessly from PCR to vector cloning regardless of the polymerase used.

Advantages & Features
  • Compatible with Blunt and TA DNA cloning: it is composed by pSpark® II and pSpark® TA DNA Cloning vectors.
  • Dual-end vector system: Eliminates the need for separate TA or blunt-end vectors.
  • High efficiency: Delivers superior transformation rates and cloning success.
  • Low background: Engineered to minimize self-ligation events.
  • Fast and simple workflow: Ready-to-use reagents for direct ligation.
  • Stable inserts: Ensures accurate and consistent clone propagation.

References

See our other cloning kits and products

Additional information

Weight 0.1 kg
Dimensions 10 × 10 × 10 cm
pack-size

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